《植物生理学报》 2015, 51(5): 709-714

火焰树的组织培养与快速繁殖

蔡月琴^*, 陆銮眉, 黄志丹, 庄培玉, 柯美玉, 王艳平

闽南师范大学生物科学与技术学院, 福建漳州363000

通信作者：蔡月琴；E-mail: 121016113@qq.com；Tel: 13960164359

摘　要：

以火焰树种子为外植体, 研究不同培养基对种子萌发、茎段增殖和生根培养的影响。结果表明, 火焰树种子经预处理后, 用70%酒精消毒30 s, 再经2% NaClO浸泡15 min的灭菌效果较好, 污染率为16.7%; 种子萌发最适培养基为MS+6-BA 2.0 mg∙L^-1+GA₃ 0.1 mg∙L^-1, 萌发率达89.1%; 较合适的茎段继代增殖培养基为WPM+6-BA 1.5 mg∙L^-1+NAA 0.2 mg∙L^-1+活性炭(AC) 0.5 g∙L^-1; 最适的生根培养基为WPM+NAA 0.2 mg∙L^-1+IBA 0.2 mg∙L^-1+AC 1 g∙L^-1, 生根率达96.7%, 每株平均生根数为6.8条。

关键词：火焰树; 种子; 组织培养; 快速繁殖

收稿：2015-04-08   修定：2015-04-29

资助：闽南师范大学园林植物生长发育与生态配置创新团队(139004)。

Tissue Culture and Rapid Propagation of Spathodea campanulata

CAI Yue-Qin^*, LU Luan-Mei, HUANG Zhi-Dan, ZHUANG Pei-Yu, KE Mei-Yu, WANG Yan-Ping

School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou, Fujian 363000, China

Corresponding author: CAI Yue-Qin; E-mail: 121016113@qq.com; Tel: 13960164359

Abstract:

Using Spathodea campanulata seeds as explants, the effects of different culture mediums on seed germination, bud multiplication and rooting were studied. The results showed that the best sterilization method for S. campanulata seeds was to soak them into 70% alcohol for 30 s and 2% NaClO for 15 min after pretreatment, with the contamination rate of 16.7%; the best medium for seed germination was MS+6-BA 2.0 mg∙L^-1+ GA₃ 0.1 mg∙L^-1, and the germination rate was up to 89.1%; the suitable medium for bud subculture and proliferation was WPM+6-BA 1.5 mg∙L^-1+NAA 0.2 mg∙L^-1+activated carbon (AC) 0.5 g∙L^-1; the optimal rooting medium was WPM+NAA 0.2 mg∙L^-1+IBA 0.2 mg∙L^-1+AC 1.0 g∙L^-1, on which the rooting rate reached 96.7% and the average number of roots was 6.8.

Key words: Spathodea campanulata; seeds; tissue culture; rapid propagation